Regulatory

Part:BBa_K3249009:Design

Designed by: Omar El-Fahmawi   Group: iGEM19_UC_Davis   (2019-10-21)


CRY2-VP64


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 723
    Illegal PstI site found at 533
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 723
    Illegal NheI site found at 1855
    Illegal PstI site found at 533
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 723
    Illegal BglII site found at 393
    Illegal BglII site found at 852
    Illegal BamHI site found at 1331
    Illegal BamHI site found at 1846
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 723
    Illegal PstI site found at 533
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 723
    Illegal PstI site found at 533
    Illegal AgeI site found at 277
    Illegal AgeI site found at 1006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 629
    Illegal BsaI.rc site found at 38
    Illegal SapI.rc site found at 146


Design Notes

Had to create a linker sequence to properly combine connect the two regions.


Source

CRY2 comes from the organism Arabidopsis thaliana.

References